Product Details | |
Product Name | Anti-5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody [RM236] |
Product Description | Rabbit monoclonal to 5-hydroxymethylcytosine (5-hmC); 5'-hmC |
Catalog No. | 31-1111-00 |
Clone Name | RM236 |
Product Data Sheet | Anti-5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody Data Sheet RM236 |
Specificity | RM236 reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA. |
Immunogen | BSA-conjugated 5-hydroxymethylcytosine. |
Application | Hydroxymethylated DNA immunoprecipitation (hMeDIP) Immunohistochemistry (IHC) Immunocytochemistry (ICC) ELISA Dot Blot (DB) |
Species Reactivity | All |
Conjugate | None |
Intended Use | Research Use Only |
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Properties | |
Form | Liquid |
Storage Instructions | Store at -20.0°C |
Stability | Stable for 1 Year at -20.0°C from date of receipt. |
Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Volume/Size | 50 ug |
Concentration | 1.0 mg/mL |
Purity | Protein A affinity purified from an animal origin–free culture supernatant |
Clone Type | Monoclonal |
Isotype | Rabbit IgG |
Usage | Dot Blot: 0.2 ug/mL – 1 ug/mL; ELISA: 0.1 ug/mL - 1 ug/mL; IHC: 0.1 ug/mL - 1 ug/mL; ICC: 0.5 ug/mL- 2 ug/mL; hMeDIP: 0.2 ug/mL- 2 ug/mL. |
References for 5-hydroxymethylcytosine (5-hmC) antibody [RM236] | J Reichmann et al. Dual spindle formation in zygotes keeps parental genomes apart in early mammalian embryos, bioRxiv; 10.1101/198275 (2017). IF; Mouse Read more |
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Product Images | |
ELISA of single stranded DNA using rabbit monoclonal |
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Dot blot of double stranded DNA using rabbit monoclonal |
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Immunohistochemical staining of formalin fixed and |
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Immunocytochemical staining of HeLa cells using |
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Direct ELISA of mouse brain genomic DNA using |
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hMeDIP was performed using anti-5-hmC antibody |