产品详情
简单介绍：
Anti-XRCC5 Antibody
详情介绍：
Overview
| Name: | Anti-XRCC5 Antibody See all XRCC5 primary antibodies
|
| Description: | Rabbit polyclonal antibody to XRCC5. |
| Applications: | WB, IHC, IF |
| Dilutions: | WB: 1:500 - 1:2000, IHC: 1:100 - 1:200, IF: 1:20 - 1:100. |
| Reactivity: | Human |
| Immunogen: | Recombinant protein of human XRCC5. |
| Protein Length: | 732 |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Unconjugated |
| Purification: | Affinity purification. |
| Product Form: | Liquid |
| Formulation: | Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol. |
| Storage: | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
Target
| Function: | Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:20383123). The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed:8621488). |
| Sequence Similarities: | Belongs to the ku80 family. |
| Post-Translational Modification: | Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity. |
| Cellular Location: | Nucleus. Nucleus > Nucleolus. Chromosome. |
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