产品名称:

Recombinant Human N-terminal Xaa-Pro-Lys N-methyltransferase 1(NTMT1)

uniprot号:

规格:

1mg 500ug 200ug 100ug 50ug 10ug

图像:

基因名称:

NTMT1

别名:

Alpha N-terminal protein methyltransferase 1AMethyltransferase-like protein 11AN-terminal R;CC1 methyltransferaseX-Pro-Lys N-terminal protein methyltransferase 1A ;NTM1A

种属:

Homo sapiens (Human)

蛋白描述:

Full Length of Mature Protein

分子量:

52.3 kDa

标签信息:

N-terminal GST-tagged

来源:

E.coli

纯度:

Greater than 85% as determined by SDS-PAGE.

缓冲液:

Tris-based buffer,50% glycerol

生物活性:

Measured by its binding ability in a functional ELISA. Immobilized CCL5 at 2 μg/ml can bind human NTMT1, the EC50 of human NTMT1 protein is 3.029-5.404 μg/ml.

参考文献:

Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000)

氨基酸序列:

TSEVIEDEKQFYSKAKTYWKQIPPTVDGMLGGYGHISSIDINSSRKFLQRFLREGPNKTGTSCALDCGAG
IGRITKRLLLPLFREVDMVDITEDFLVQAKTYLGEEGKRVRNYFCCGLQDFTPEPDSYDVIWIQWVIGHL
TDQHLAEFLRRCKGSLRPNGIIVIKDNMAQEGVILDDVDSSVCRDLDVVRRIICSAGLSLLAEERQENLP
DEIYHVYSFALR

简介:

Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.

蛋白储存:

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

表达区域:

2-223aa

使用注意:

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

研究领域:

Cell Biology