产品类型:

Enzyme

产品名称:

Recombinant Human NAD-dependent malic enzyme, mitochondrial protein(ME2) (Active)

uniprot号:

规格:

5μg 25μg 100μg 250μg ￥620

图像:

基因名称:

ME2

别名:

NAD-ME, Malic enzyme 2

种属:

Homo sapiens (Human)

蛋白描述:

Full Length of Mature Protein

PubMed ID:

1993674; 11401430; 14702039; 16177791; 15489334; 12665801; 19608861; 21269460; 24275569; 10467136; 10700286; 12121650; 12962632

分子量:

64.4 kDa

标签信息:

C-terminal 6xHis-tagged

来源:

E.Coli

纯度:

>95% as determined by SDS-PAGE and HPLC.

缓冲液:

0.2μm filtered PBS, pH 7.4. ,lyophilized

内毒素:

Less than 1.0 EU/μg as determined by LAL method.

生物活性:

Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 ± 0.6 mM

Uniprot登录名:

MAOM_HUMAN

氨基酸序列:

MLHIKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMG
IQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKA
VVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMGLYQKRDR
TQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTAAVALAGLLAAQKV
ISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTH
SAPESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSNPTAQAECTAEEAYTLT
EGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIFPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEEL
AQGRLYPPLANIQEVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYEWPESAS
SPPVITE+HHHHHH

表达区域:

19-584aa

研究领域:

Signal Transduction