产品类型:Tag/Control Antibodies
产品名称:
规格:
免疫原:
HHHHHH(6x His) synthetic peptide conjugate to KLH
宿主:
Mouse
应用范围:
ELISA, WB; Recommended dilution: WB:1:500-1:5000
背景:
A HIS tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the HIS codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
克隆类型:
Monoclonal
抗体亚型:
IgG2a
纯化方式:
>95%,Protein G purified
标记方式:
Non-conjugated
保存缓冲液:
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
产品提供形式:
Liquid
储存条件:
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
图片:
All lanes :Mouse Anti-6*his monoclonal antibody at 1ug/ml
Western blot analysis of 6*his Epitope Tag was performde by loading various amounts of lysates containing different HIS tagged
Recombinant proteins per well onto a 15% Tris-HCL polyacrylamide gel.
Line 1: FABP3 insect cell lysate
Line 2: TK1 insect cell lysate
Line 3: cagA insect cell lysate
Line 4: NGAL insect cell lysate
Line 5: IGFBP1 293T cell lysate
Line 6: TRY2 293T cell lysate
All lanes : Recombinant DST Protein at 30ng/ml
Line 1: Mouse Anti-his monoclonal antibody at 1:64000
Line 2: Mouse Anti-his monoclonal antibody at 1:32000
Line 3: Mouse Anti-his monoclonal antibody at 1:16000
Line 4: Mouse Anti-his monoclonal antibody at 1:8000
Line 5: Mouse Anti-his monoclonal antibody at 1:4000
Observed band size : 35kd
Predicted band size : 35kd
别名:
6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
