产品类型:

Tag/Control Antibodies

产品名称:

GFP Monoclonal Antibody

规格:

100μg 50μg ￥1,200

免疫原:

Recombinant GFP Protein

宿主:

mouse

应用范围:

ELISA,WB,IP;Recommended dilution:WB:1:500-1:5000,IP:1:200-1:2000

背景:

The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. The protein is in the shape of a cylinder, comprising 11 strands of beta-sheet with an alpha-helix inside and short helical segments on the ends of the cylinder. Inward-facing sidechains of the barrel induce specific cyclization reactions in the tripeptide Ser65-Tyr66-Gly67 that lead to chromophore formation. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has always been used as a universal reporter in a broad range of heterologous living cells and organisms. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms.

克隆类型:

monoclonal

抗体亚型:

IgG2b

纯化方式:

>95%,Protein G purified

标记方式:

Non-conjugated

保存缓冲液:

Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

产品提供形式:

liquid

储存条件:

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

图片:

WB: Mouse Anti-GFP monoclonal antibody at 0.1ug/ml
Line 1:HEK-293 cell lysate
Line 2:GFP transfected HEK-293 cell lysate
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 30kd
Observed band size : 30kd

 

IP:Result of anti-GFP-tag Monoclonal antibody
Line 1:Control IgG
Line 2:Precipitateing GFP-tagged fusion protein
Line 3:GFP Transfected HEK-293 cells lysate
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 30kd
Observed band size : 30kd

别名:

CFP, eGFP, eYFP, GFP, GFP tag, YFP