产品类型:

Tag/Control Antibodies

产品名称:

Flag Tag Monoclonal Antibody

规格:

100μg 50μg ￥1,200

免疫原:

DYKDDDDK(FLAG) synthetic peptide conjugate to KLH

宿主:

mouse

应用范围:

ELISA,WB,IF,IP;Recommended dilution:WB:1:500-1:5000,IF:1:50-1:200,IP:1:200-1:2000

背景:

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

克隆类型:

monoclonal

抗体亚型:

IgG2a

纯化方式:

>95%,Protein G purified

标记方式:

Non-conjugated

保存缓冲液:

Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

产品提供形式:

liquid

储存条件:

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

图片:

Western Immunoblot analysis of randomly picked 7 differend DDK-tagged overexpressio lysates with CSB-MA000021M0m at 1:2000

IF:Hela cells transfected with DDK-tagged CD146 ORF cDNA clone were immunostained with M0002 antibody,and then colored in greed with FITC conjugated Secondary antibody(1:2000).The nuclei(blue) were counterstained with DAPI.

IP-WB:Result of anti DDK-tag Monoclonal antibody
Lane 1:DDK-tagged fusion protein
Transfected HEK-293 cells lysate
Lane 2:Elution
Lane 3:Precipitateing DDK-tagged fusion protein
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 40kd
Observed band size : 40kd

别名:

3X Flag, DDK tag, DYKDDDDK, Flag, Flag Tag