产品详情
简单介绍：
One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 50nm gold nanourchins. Contains enough reagents for conjugation of 100ug of protein.
详情介绍：
Product Details
Cytodiagnostics 50nm NHS-activated Gold NanoUrchins Conjugation Kits have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to gold nanourchins.
The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanourchins is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester gold nanourchins supplied in the kit.
Kit Components
- 50nm NHS-Activated Gold NanoUrchins (lyophilized)
- Protein Resuspension Buffer
- Reaction Buffer
- Quencher Solution
Features & Benefits
- Results in covalently bound ligand and more stable conjugate.
- Fast and convenient one-step conjugation reaction with no pre-activation requirements.
- Spacer between the gold surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
- Precisely engineering surface coating that reduces non-specific protein binding in biological assays.
Gold Nanoparticle Specifications
Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between gold surface and NHS-group)
Core diameter: 50nm
Concentration: OD=20 (when dissolved to a final volume of 100ul)
Particles per ml: ~7.02E+11 (when dissolved to a final volume of 100ul)
Absorbance (lambda max): 585nm
Diameter | Peak SPR Wavelength (nm) | NPS/ml* | Wt. Conc (mg/ml)* | Molar Ext (M-1cm-1) | Size Dispersity (+/-nm) | Particle Volume (nm3)# | Surface Area (nm2)# | Surface/ Volume Ratio# | Particle Mass (g) | Molar Mass (g/mol) | Molar Concentration* |
50 | 585 | 3.51E+10 | 4.45E-02 | 1.72E+10 | <8% | 6.54E+04 | 7.85E+03 | 0.12 | 1.27E-15 | 7.64E+08 | 5.83E-11 |
| *All concentrations as indicated at an optical density of 1 (OD=1), # Data is approximated based upon a spherical nanoparticle. | |||||||||||
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