RT4

RT4 

 RT4 (ATCC® HTB-2™)
Organism  Homo sapiens, human 
Tissue  urinary bladder
Product Format  frozen 
Morphology  epithelial 
Culture Properties  adherent 
Biosafety Level  1 
Disease  transitional cell papilloma 
Age  63 years 
Gender  male 
Ethnicity  Caucasian 
Applications  This cell line is a transfection host.
Storage Conditions  liquid nitrogen vapor phase 
Karyotype  modal number = 49; range = 42 to 55. The cell line is aneuploid human male, with chromosome counts in the near-diploid range. However, the near-tetraploid population may become predominant within very few passages. The 48 and 49 chromosome karyotypes had a single X and a single Y chromosome. Three of the karyotypes at higher ploidy had two X chromosomes and a single Y chromosome. Four marker chromosomes were identified: del(6)(q21), del(10)(p11), 14p+, 12p+. Marker chromosome M4 was not seen in the karyotypes prepared from higher ploidy metaphases. 
Clinical Data  63 years
Caucasian
male
Antigen Expression  Antigen expression: HLA A25(10), A3, B12, Cw3; Blood Type O 
Tumorigenic  Yes 
Effects  Yes, in cheek pouch of steroid-treated hamsters
Comments  A contamination with Mycoplasma orale was cured in October 1971.

Complete Growth Medium  The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
 
Subculturing  Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation  Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions  Temperature: 37°C

STR Profile  Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8
D16S539: 9
D5S818: 11,12
D7S820: 9,12
THO1: 9,9.3
TPOX: 8,11
vWA: 14,17
Isoenzymes  AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 1-2
PGM3, 1-2

Name of Depositor  C Rigby, LM Franks 
References  O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.

O'Toole C, et al. Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int. J. Cancer 10: 77-91, 1972. PubMed: 4196436

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572

Lasfargues EY, et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035

Rigby CC, Franks LM. A human tissue culture cell line from a transitional cell tumour of the urinary bladder: growth, chromosone pattern and ultrastructure. Br. J. Cancer 24: 746-754, 1970. PubMed: 5503601

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212
 

References  O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.

O'Toole C, et al. Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int. J. Cancer 10: 77-91, 1972. PubMed: 4196436

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572

Lasfargues EY, et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035

Rigby CC, Franks LM. A human tissue culture cell line from a transitional cell tumour of the urinary bladder: growth, chromosone pattern and ultrastructure. Br. J. Cancer 24: 746-754, 1970. PubMed: 5503601

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212
 

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