CCL-89 AtT-20 小鼠垂体瘤细胞 ATCC® Number: CCL-89™ Designations: AtT-20 Depositors: G Sato Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Organism: Mus musculus (mouse) Morphology: small rounded cells
Source: Organ: pituitary Strain: LAF1 Disease: tumor Cellular Products: adrenocorticotropic hormone (ACTH) Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Isolation: Isolation date: October, 1966 Applications: transfection host (Roche FuGENE® Transfection Reagents) Virus Susceptibility: Vesicular stomatitis virus Human poliovirus 1 Reverse Transcript: positive Comments: Tested and found negative for ectromelia virus (mousepox). Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%. Temperature: 37.0°C Growth Conditions: The cells do not attach but aggregate to form clusters; the clusters are viable. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Subculturing: Protocol: Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. Proliferation is enhanced by incubation on a shaking platform. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Preservation: Freeze medium: culture medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004 recommended serum:ATCC 30-2020 recommended serum:ATCC 30-2040 derivative:ATCC CRL-1795 References: 23251: Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682 26257: Furth J, et al. ACTH secreting transplantable pituitary tumors. Proc. Soc. Exp. Biol. Med. 84: 253-254, 1953. PubMed: 13121002 28712: Tabernero C, et al. Identification of an RNA sequence within an intracisternal-A particle element able to replace Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1. J. Virol. 71: 95-101, 1997. PubMed: 8985327 33043: Gamby C, et al. Growth-associated protein-43 (GAP-43) facilitates peptide hormone secretion in mouse anterior pituitary AtT-20 cells. J. Biol. Chem. 271: 10023-10028, 1996. PubMed: 8626556
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