CCL-244 HCT-8 人回盲肠癌细胞 ATCC® Number: CCL-244™ Designations: HCT-8 [HRT-18] Depositors: A Sobrero Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Homo sapiens (human) Morphology: epithelial
Source: Organ: colon Disease: ileocecal colorectal adenocarcinoma Cellular Products: carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days; alkaline phosphatase; keratin Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Tumorigenic: Yes Reverse Transcript: negative DNA Profile (STR): Amelogenin: X,Y CSF1PO: 12 D13S317: 8,11 D16S539: 12,13 D5S818: 13 D7S820: 10,12,11.3 THO1: 7,9.3 TPOX: 8,11 vWA: 18,19 Isoenzymes: AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1 Age: 67 years Gender: male Comments: The HCT-8 line is identical to the HRT-18 cell line. The cells are positive for keratin by immunoperoxidase staining. Propagation: ATCC complete growth medium: RPMI 1640 medium with sodium pyruvate (1 mM); 90%; horse serum, 10% Temperature: 37.0°C Subculturing: Protocol: Remove medium, rinse cell sheet twice with 0.25% trypsin, 0.02% EDTA in Hanks' BSS and incubate at 37C with the trypsin, EDTA for 10 to 15 minutes. Remove trypsin, EDTA, disperse cells in fresh medium, centrifuge, resuspend cells in medium and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days References: 1052: Tompkins WA, et al. Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J. Natl. Cancer Inst. 52: 1101-1110, 1974. PubMed: 4826581 26064: Nelson-Rees WA, et al. Distinctive banded marker chromosomes of human tumor cell lines. Int. J. Cancer 16: 74-82, 1975. PubMed: 1058173 32376: White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293
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