HeLa 229 人宫颈癌细胞 ATCC® Number: CCL-2.1™ Price: $323.00 Designations: HeLa 229 Depositors: JT Syverton Biosafety Level: 2 [Cells contain human papilloma virus (HPV-18) ] Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent (The line can be readily adapted to growth in suspension) Organism: Homo sapiens (human) Morphology: epithelial
Source: Organ: cervix Disease: adenocarcinoma Cellular Products: keratin Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Virus Susceptibility: Human adenovirus 7 Vesicular stomatitis virus Human poliovirus 1 Human poliovirus 2 Human poliovirus 3 Reverse Transcript: negative DNA Profile (STR): Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 Isoenzymes: G6PD, A Age: 31 years Gender: female Ethnicity: Black HeLa Markers: Y Comments: Relatively resistant to polioviruses. The cells are positive for keratin by immunoperoxidase staining. Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
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