CRL-1830 Hepa 1-6 小鼠肝癌细胞 ATCC® Number: CRL-1830™ Designations: Hepa 1-6 Depositors: GJ Darlington Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Mus musculus (mouse) Morphology: epithelial
Source: Organ: liver Strain: C57L Disease: hepatoma Cellular Products: albumin, alpha fetoprotein (AFP, alpha-fetoprotein); albumin; alpha 1 antitrypsin (alpha-1-antitrypsin); amylase Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Applications: transfection host (Roche FuGENE® Transfection Reagents) Comments: This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse. Tested and found negative for ectromelia virus (mousepox). Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: The cells can also be propagated in a serum free medium consisting of 3 parts Dulbecco's modified Eagle's medium, 1 part Waymouth's MAB 87/3 medium and 30 nM selenium. Subculturing: Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Two to three times weekly Preservation: Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101 Cell culture tested DMSO:ATCC 4-X References: 1596: Darlington GJ. Liver cell lines. Methods Enzymol. 151: 19-38, 1987. PubMed: 3431441 1597: Darlington GJ, et al. Expression of liver phenotypes in cultured mouse hepatoma cells. J. Natl. Cancer Inst. 64: 809-819, 1980. PubMed: 6102619
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