CCL-186 IMR-90 人胚肺成纤维细胞 ATCC® Number: CCL-186™ Designations: IMR-90 Depositors: WW Nichols Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Homo sapiens (human) Morphology: fibroblast
Source: Organ: lung Disease: normal Cell Type: fibroblast Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Applications: transfection host (Nucleofection technology from Lonza) Virus Susceptibility: Human poliovirus 1 Human poliovirus 2 Varicella-Zoster Herpes simplex virus 1 Herpes simplex virus 2 Human poliovirus 3 Vaccinia virus Human herpesvirus 5 Vesicular stomatitis virus Reverse Transcript: negative DNA Profile (STR): Amelogenin: X CSF1PO: 11,13 D13S317: 11,13 D16S539: 10,13 D5S818: 12,13 D7S820: 9,12 THO1: 8,9.3 TPOX: 8,9 vWA: 16,19 Cytogenetic Analysis: normal human female; diploid; stable Isoenzymes: G6PD, B Age: 16 weeks gestation Gender: female Ethnicity: Caucasian Comments: The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. [22381] The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains. The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. [22381] Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Subculturing: Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: Every 3 to 4 days Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 References: 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339 32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642 33041: Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564 Related Links NCBI Entrez Search Make a Deposit Frequently Asked Questions Material Transfer Agreement Technical Support Related Cell Culture Products
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