CCL-246 KG-1 人白血病细胞 ATCC® Number: CCL-246™ Designations: KG-1 Depositors: DW Golde Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: suspension Organism: Homo sapiens (human) Morphology: myeloblast
Source: Organ: bone marrow Disease: acute myelogenous leukemia Cellular Products: HLA DR Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Applications: transfection host (technology from amaxa) Reverse Transcript: negative Antigen Expression: HLA A30, A31, B35, Cw4 DNA Profile (STR): Amelogenin: X,Y CSF1PO: 7 D13S317: 11,12 D16S539: 10,11 D5S818: 13 D7S820: 8,10 THO1: 7,8 TPOX: 7,9 vWA: 14,19 Cytogenetic Analysis: The stemline chromosome number is near-diploid, with the 2S component occurring at 1.8%. Five markers (constitutive markers) were common to most, if not all, metaphases analyzed. Modal chromosome number is 47 (or 46 plus a small metacentric chromosome which is smaller than the G1 group chromosome). Normal chromosomes 5, 7, 8, 12 and 17 were monosomic, and others were disomic. The Y chromosome is detected in the Q-banded preparations. Isoenzymes: AK-1, 0 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1-2 PGM3, 0 Age: 59 years Gender: male Ethnicity: Caucasian Comments: The KG-1 cell line was derived by H.P. Koeffler and D.W. Golde. A bone marrow aspirate was obtained from a 59-year-old Caucasian male with erythroleukemia that evolved into acute myelogenous leukemia. [1056] KG-1 cells spontaneously differentiate to granulocyte and macrophage like cells. They show a good response to colony stimulating factor (CSF). [1056] The line is EBNA negative (EBNA-). Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Temperature: 37.0°C Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 (5) viable cells/ml. Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml. Do not allow to exceed 2 X 10(6) cells/ml. Medium Renewal: Twice per week Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020 References: 867: Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765 1056: Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682 26069: . . Blood 54: 174a, 1979. 33055: Penrose JF, et al. Molecular cloning of the gene for human leukotriene C4 synthase. J. Biol. Chem. 271: 11356-11361, 1996. PubMed: 8626689 Related Links NCBI Entrez Search Make a Deposit Frequently Asked Questions Material Transfer Agreement Technical Support Related Cell Culture Products
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