CCL-219 L1210 小鼠白血病细胞 ATCC® Number: CCL-219™ Designations: L1210 Depositors: EM Jensen Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: suspension Organism: Mus musculus (mouse) Morphology: lymphoblast
Source: Disease: lymphocytic leukemia Strain: DBA subline 212 Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Tumorigenic: Yes Reverse Transcript: positive Cytogenetic Analysis: modal number = 40; range = 32 to 80. Mouse karyotype with a diploid stemline number of 40 chromosomes. Age: 8 months Gender: female Comments: Tested and found negative for ectromelia virus (mousepox). The line was established from a tumor in female #234 that developed following skin paintings with 0.2% methylcholanthrene in ether. [22513] Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%. Temperature: 37.0°C Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 viable cells/ml. Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 4 days Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase Doubling Time: 8 to 10 hrs Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2040 References: 22513: . . J. Natl. Cancer Inst. 10: 179-192, 1949. 26055: . . J. Natl. Cancer Inst. 36: 405-421, 1966. 26056: . . Cancer Chemother. Rep. 51: 451-453, 1967. 32923: Young SW, et al. Gadolinium(III) texaphyrin: a tumor selective radiation sensitizer that is detectable by MRI. Proc. Natl. Acad. Sci. USA 93: 6610-6615, 1996. PubMed: 8692865 Related Links NCBI Entrez Search Make a Deposit Frequently Asked Questions Material Transfer Agreement Technical Support Related Cell Culture Products
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