CCL-7 LLC-MK2 恒河猴肾细胞 ATCC® Number: CCL-7™ Designations: LLC-MK2 Original Depositors: RN Hull Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Macaca mulatta (monkey, rhesus) Morphology: epithelial
Source: Organ: kidney Disease: normal Cellular Products: plasminogen activator Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Virus Susceptibility: poliovirus 1; coxsackievirus B4; bovine viral diarrhea (BVD) virus Reverse Transcript: negative Age: Comments: The serum used in the medium should be pre-tested for good quality. Some lots of horse serum do support satisfactory growth. Adaptation to other types of sera generally results in slow growth with a prolonged lag in growth. Propagation: ATCC complete growth medium: Medium 199 (with 1.68 g/L bicarbonate), 99%; horse serum, 1% Temperature: 37.0°C Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks. Preservation: Culture medium, 95%; DMSO, 5% Related Products: recommended serum:ATCC 30-2040 References: 22310: . . Anat. Rec. 124: 490, 1956. 26049: Hull RN, et al. Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research. J. Exp. Med. 115: 903-918, 1962. PubMed: 14449901 26065: Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902
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