CRL-1825 P19 小鼠畸胎瘤细胞 ATCC® Number: CRL-1825™ Designations: P19 Depositors: MW McBurney Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Mus musculus (mouse) Morphology: epithelial
Source: Organ: embryo Strain: C3H/He Disease: teratocarcinoma; embryonal carcinoma Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Applications: transfection host (Nucleofection technology from Lonza) Cytogenetic Analysis: n = 40; XY, n = 40; XY [22702] Gender: male Comments: The P19 line was derived from an embryonal carcinoma induced in a C3H/He mouse. [22702] The line can be cloned at high efficiency in medium containing 0.1 mM 2-mercaptoethanol. [22702] The cells are pluripotential. The cell can be induced to differentiate into neural and glial like cells in the presence of 500 nM retinoic acid. [22492] In the presence of 0.5% to 1.0% dimethylsulfoxide (DMSO) the cells differentiate to form cardiac and skeletal muscle-like elements, but do not form neural or glial like cells. [22913] In the presence of both DMSO and retinoic acid, the cells differentiate as in the presence of retinoic acid alone. [22913] Propagation: ATCC complete growth medium: The base medium for this cell line is Alpha Minimum Essential Medium with ribonucleosides and deoxyribonucleosides. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 7.5%; fetal bovine serum to a final concentration of 2.5%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% Subculturing: Protocol: Do not allow the cells to become confluent.
Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:10 every 2 to 3 days is recommended Medium Renewal: Add fresh medium at least every 48 hours Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase Related Products: recommended serum:ATCC 30-2020 recommended serum:ATCC 30-2030 References: 21632: Bennicelli JL, et al. Mechanism for transcriptional gain of function resulting from chromosomal translocation in alveolar rhabdomyosarcoma. Proc. Natl. Acad. Sci. USA 93: 5455-5459, 1996. PubMed: 8643596 22492: Jones-Villeneuve EM, et al. Retinoic acid induces embryonal carcinoma cells to differentiate into neurons and glial cells. J. Cell Biol. 94: 253-262, 1982. PubMed: 7107698 22702: McBurney MW, Rogers BJ. Isolation of male embryonal carcinoma cells and their chromosome replication patterns. Dev. Biol. 89: 503-508, 1982. PubMed: 7056443 22913: McBurney MW, et al. Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line. Nature 299: 165-167, 1982. PubMed: 7110336
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