TIB-9 P3X63Ag8 小鼠骨髓瘤细胞 ATCC® Number: TIB-9™ Designations: P3X63Ag8 Depositors: G Kohler, C Milstein Isotype: IgG1; kappa light chain Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: suspension Organism: Mus musculus (mouse) Morphology: lymphoblast
Source: Disease: plasmacytoma; myeloma Strain: BALB/c Cell Type: B lymphocyte; Cellular Products: immunoglobulin; monoclonal antibody Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Antigen Expression: H-2d Cytogenetic Analysis: hyperdiploid; modal number = 65 Comments: This line was derived from the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma). The line is resistant to 0.1 mM 8-azaguanine and die in HAT medium. The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity. Tested and found negative for ectromelia virus (mousepox). Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing: Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. References: 1042: Koprowski H, et al. Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proc. Natl. Acad. Sci. USA 74: 2985-2988, 1977. PubMed: 268647 1112: Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495-497, 1975. PubMed: 1172191 22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579 22173: Kohler G, et al. Fusion of T and B cells. Somatic Cell Genet. 3: 303-312, 1977. PubMed: 305123 22347: Kohler G, Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6: 511-519, 1976. PubMed: 825377 23366: Sato JD, et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed: 3209588 26333: Ramasamy R, et al. Possible role for the Fc receptor on B lymphocytes. Nature 249: 573-574, 1974. PubMed: 4545851 26334: Lemke H, et al. Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse. Nature 271: 249-251, 1978. PubMed: 74805 26335: . . Eur. J. Immunol. 3: 135-140, 1973. 26336: Secher DS, et al. Spontaneous mutation in tissue culture-chemical nature of variant immunoglobulin from mutant clones of MOPC 21. FEBS Lett. 37: 311-316, 1973. PubMed: 4763339
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