CCL-136 RD 人恶性胚胎横纹肌瘤细胞 ATCC® Number: CCL-136™ Designations: RD Depositors: RM McAllister Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Homo sapiens (human) Morphology: spindle cells and large multinucleated cells
Source: Tissue: muscle Disease: rhabdomyosarcoma Cellular Products: myoglobin myosin ATPase Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 3 to 4 days Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 References: 21447: American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992. 22219: McAllister RM, et al. Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer 24: 520-526, 1969. PubMed: 4241949 22238: Chen TR, et al. Cell identity resolved. Nature 340: 106, 1989. PubMed: 2739733 22929: Stratton MR, et al. Misidentified cell. Nature 337: 311-312, 1989. PubMed: 2911385 23221: Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033 26001: . . J. Virol. 1: 326, 1967.
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