HTB-77 SK-OV-3 人卵巢癌细胞

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产品名称：SK-OV-3 人卵巢癌细胞
产品型号：HTB-77
产品展商：美国标准生物品收藏中心（ATCC）
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简单介绍

HTB-77 SK-OV-3 人卵巢癌细胞，ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件

产品描述

HTB-77 SK-OV-3 人卵巢癌细胞

ATCC^® Number:

HTB-77™

Price:

$272.00

Designations:	SK-OV-3 [SKOV-3]
Depositors:	G Trempe, LJ Old
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens (human)
Morphology:	epithelial
Source:	Organ: ovary Disease: adenocarcinoma Derived from metastatic site: ascites
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions:
Tumorigenic:	Yes
Antigen Expression:	Blood Type B; Rh+
DNA Profile (STR):	Amelogenin: X CSF1PO: 11 D13S317: 8,11 D16S539: 12 D5S818: 11 D7S820: 13,14 THO1: 9,9.3 TPOX: 8,11 vWA: 17,18
Cytogenetic Analysis:	This is a hypodiploid human cell line. The modal chromosome number was 43, occurring in 63.3% of cells. The range was 42 to 45. The rate of higher ploidies was 32%. The del(1)(q21), der(13)t(1;?;13) (q11;?;q34), der(11)t(11;?) (q12), del(10)(q22) and 3 other marker chromosomes were common to most cells, and 3 others were found only in some cells. One N11 had the HSR segment from p11 to the distal end. The normal N10, N12, N15, N17 and N19 were absent. Others were either single or paired. There were from 1 to 6 rearranged and unassignable chromosomes. The X chromosome was either single or paired.
Isoenzymes:	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1-2 PGM3, 1
Age:	64 years
Gender:	female
Ethnicity:	Caucasian
Comments:	SK-OV-3 cells are resistant to tumor necrosis factor and to several cytotoxic drugs including diphtheria toxin, cis-platinum and adriamycin.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week
Preservation:	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007 recommended serum:ATCC 30-2020
References:

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SK-OV-3 人卵巢癌细胞