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HOS 人骨肉瘤细胞

HOS 人骨肉瘤细胞
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  • 产品名称：HOS 人骨肉瘤细胞
  • 产品型号：CRL-1543
  • 产品展商：美国标准生物品收藏中心（ATCC）
  • 产品文档：无相关文档
简单介绍
HOS 人骨肉瘤细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！
产品描述
HOS 人骨肉瘤细胞
ATCC® Number: CRL-1543™    Price: $323.00
Designations: HOS
Depositors:  JS Rhim
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: mixed, fibroblast and epithelial like cells
Source: Organ: bone
Disease: osteosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
Isoenzymes: G6PD, B
Age: 13 years
Gender: female
Ethnicity: Caucasian
Comments: HOS cells exhibit flat morphology, low saturation density, low plating efficiency in soft agar and are sensitive to chemical and viral transformation.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
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