产品 资讯
请输入产品名称
噪声分析仪 纺织检测仪器 Toc分析仪 PT-303红外测温仪 转矩测试仪 继电保护试验仪 定氮仪
首页 产品 知识 专题 词条 品牌 资料 展会 成功案例 网上展会
词多 效果好 就选易搜宝！
首页 >>> 产品目录 >>> ATCC细胞、细胞株、肿瘤细胞 >>> 其它动物细胞系目录
仪表展览网 >>> 展馆展区 >>> 塑料及制品 >>> PE >>> CRL-2285 Super Tube 狗肾细胞 > CRL-2285 Super Tube 狗肾细胞

产品资料

CRL-2285 Super Tube 狗肾细胞

CRL-2285 Super Tube 狗肾细胞
  • 如果您对该产品感兴趣的话，可以
  • 产品名称：CRL-2285 Super Tube 狗肾细胞
  • 产品型号：CRL-2285
  • 产品展商：美国标准生物品收藏中心（ATCC）
  • 产品文档：无相关文档
简单介绍
CRL-2285 Super Tube 狗肾细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和Z优培养条件！
产品描述
CRL-2285 Super Tube 狗肾细胞
ATCC® Number: CRL-2285™     
Designations: Super Tube
Depositors:  RJ Klebe
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Canis familiaris
Morphology: epithelial
Source: Organ: kidney
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Age:
Gender: female
Comments: Super Tube is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal ***** female cocker spaniel.
Super tube forms large numbers of tubules when maintained at high cell density (cells are reported to form tubules within 3 days when plated at 7 x 10exp5 cells/well of a 24 well plate).
For tubule formation, the cells should be fed twice a day by a complete medium change.
Super Tube has a much lower resting level of cyclic-AMP and responds to forskolin stimulation of adenylate cyclase less readily than the original cell line; it also has a lower transepithelal resistance than both Super Dome (ATCC CRL- 2286) and MDCK.
Propagation: ATCC complete growth medium: These cells are grown in a medium containing a 1:1 mixture of Dulbeccos Modified Eagles Medium and Hams F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10 fetal bovine serum.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25 trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95; DMSO, 5
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
parental cell line:ATCC CCL-34
derived from same cell line:ATCC CRL-2286
References: 38915: Klebe RJ, et al. Cyclic-AMP deficient MDCK cells form tubules. J. Cell. Biochem. 59: 453-462, 1995. PubMed: 8749715
产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!
  • 温馨提示：为规避购买风险，建议您在购买前务必确认供应商资质与产品质量。
  • 免责申明：以上内容为注册会员自行发布，若信息的真实性、合法性存在争议，平台将会监督协助处理，欢迎举报
产品留言
标题
内容
联系人
联系电话
电子邮件
公司名称
联系地址
验证码
点击换一张
注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

沪公网安备 31010902002429号