CRL-1671 RL95-2 人**内膜癌细胞

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产品名称：CRL-1671 RL95-2 人**内膜癌细胞
产品型号：CRL-1671
产品展商：美国标准生物品收藏中心（ATCC）
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简单介绍

CRL-1671 RL95-2 人**内膜癌细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！

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CRL-1671 RL95-2 人**内膜癌细胞

ATCC^® Number:

CRL-1671™

Price:

$323.00

Designations:	RL95-2
Depositors:	DL Way
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens (human)
Morphology:	epithelial
Source:	Organ: uterus Tissue: endometrium Disease: carcinoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors:	estrogen receptor, positive
DNA Profile (STR):	Amelogenin: X CSF1PO: 10,11 D13S317: 8,12 D16S539: 11,13 D5S818: 10,11 D7S820: 10 THO1: 9,9.3 TPOX: 8 vWA: 16,20
Age:	65 years
Gender:	female
Ethnicity:	Caucasian
Comments:	The cells possess alpha keratin, well defined junctional complexes, tonofilaments and surface microvilli.
Propagation:	ATCC complete growth medium: A 1:1 mixture of Dulbeccos modified Eagles medium and Hams F12 medium with 10 mM HEPES and 2.0 g/L sodium bicarbonate supplemented with 0.005 mg/ml insulin and 10 fetal bovine serum Temperature: 37.0°C
Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25 trypsin, 0.03 EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation:	culture medium 95; DMSO, 5
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020
References:	2151: Way DL, et al. Characterization of a new human endometrial carcinoma (RL95-2) established in tissue culture. In Vitro 19: 147-158, 1983. PubMed: 6339371 22666: Cohen LA. Isolation and characterization of a serially cultivated, neoplastic, epithelial cell line from the N-nitrosomethylurea induced rat mammary adenocarcinoma. In Vitro 18: 565-575, 1982. PubMed: 7118137 29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 32565: Yang N, et al. Estrogen-related receptor, hERR1, modulates estrogen receptor-mediated response of human lactoferrin gene promoter. J. Biol. Chem. 271: 5795-5804, 1996. PubMed: 8621448

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CRL-1671 RL95-2 人**内膜癌细胞