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CRL-5908 NCI-H1975 人肺腺癌细胞

CRL-5908 NCI-H1975 人肺腺癌细胞
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  • 产品名称：CRL-5908 NCI-H1975 人肺腺癌细胞
  • 产品型号：CRL-5908
  • 产品展商：美国标准生物品收藏中心（ATCC）
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简单介绍
CRL-5908 NCI-H1975 人肺腺癌细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！
产品描述
CRL-5908 NCI-H1975 人肺腺癌细胞
ATCC® Number: CRL-5908™    Price:
Designations: NCI-H1975 [H-1975, H1975]
Depositors:  AF Gazdar, JD Minna
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
CRL-5908 NCI-H1975 人肺腺癌细胞
Source: Organ: lung
Disease: adenocarcinoma; non-small cell lung cancer
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 10,13
D16S539: 9,12
D5S818: 11,12
D7S820: 8,11
THO1: 7
TPOX: 8,11
vWA: 18
Gender: female
Comments: The line was established in July 1988.
The tissue donor was a non-smoker.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse the monolayer with fresh 0.25 trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: 1:3 to 1:6
Medium Renewal: two to three times weekly
Preservation: Freeze medium: culture medium, 95; DMSO, 5
Storage temperature: liquid nitrogen vapor phase
References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..
90471: Sordella R, et al. Gefitinib-sensitizing EGFR mutations in lung cancer activate anti-apoptotic pathways. Science 305: 1163-1167, 2004. PubMed: 15284455
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