CRL-1547 MNNG HOS Cl 5 人骨肉瘤细胞

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产品名称：CRL-1547 MNNG/HOS Cl #5 人骨肉瘤细胞
产品型号：CRL-1547
产品展商：美国标准生物品收藏中心（ATCC）
产品文档：无相关文档

简单介绍

CRL-1547 MNNG/HOS Cl #5 人骨肉瘤细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和Z优培养条件！

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CRL-1547 MNNG/HOS Cl #5 人骨肉瘤细胞

ATCC^® Number:

CRL-1547™

Price:

Designations:	MNNG/HOS Cl #5 [R-1059-D]
Depositors:	JS Rhim
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens (human)
Morphology:
Source:	Organ: bone Disease: osteosarcoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic:	Yes
DNA Profile (STR):	Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 10,13 D5S818: 13 D7S820: 11,12 THO1: 6 TPOX: 8,11 vWA: 18
Isoenzymes:	G6PD, B
Age:	13 years
Gender:	female
Ethnicity:	Caucasian
Comments:	This line was derived from HOS (ATCC CRL-1543) by transformation with 0.01 mcg/ml MNNG (a carcinogenic nitrosamine). The cells exhibit a high saturation density, a high plating efficiency in soft agar and are tumorigenic in nude mice.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended Medium Renewal: 2 to 3 times per week Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 2 to 5 days.
References:	22895: Rhim JS, et al. Transformation of human cells in culture by N-methyl-N'-nitro-N- nitrosoguanidine. Nature 256: 751-753, 1975. PubMed: 1057046 26084: Rhim JS, et al. Characterization of human cells transformed in vitro by N-methyl-N'- nitro-N-nitrosoguanidine. Int. J. Cancer 19: 505-510, 1977. PubMed: 265298 32925: Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224

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CRL-1547 MNNG/HOS Cl #5 人骨肉瘤细胞