CRL-1973 NTERA-2 人恶性多发性畸胎瘤细胞

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产品名称：CRL-1973 NTERA-2 人恶性多发性畸胎瘤细胞
产品型号：CRL-1973
产品展商：美国标准生物品收藏中心（ATCC）
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CRL-1973 NTERA-2 人恶性多发性畸胎瘤细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和Z优培养条件！

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CRL-1973 NTERA-2 人恶性多发性畸胎瘤细胞

ATCC^® Number:

CRL-1973™

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Designations:	NTERA-2 cl.D1 [NT2/D1]
Depositors:	PW Andrews
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens (human)
Morphology:	epithelial-like,differentiation changes phenotype
Source:	Organ: testis Disease: malignant pluripotent embryonal carcinoma Derived from metastatic site: lung
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:	transfection host (Roche FuGENE® Transfection Reagents technology from amaxa)
Virus Susceptibility:	RA and HMBA TREATED CELLS: human cytomegalovirus (HCMV); human immunodeficiency virus (HIV-1, HTLV-III)
Virus Resistance:	UNTREATED CELLS: human cytomegalovirus (HCMV); human immunodeficiency virus (HIV-1, HTLV-III)
DNA Profile (STR):	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 13 D16S539: 11,12,13 D5S818: 9,12 D7S820: 10,12 THO1: 9.3 TPOX: 8 vWA: 18,19
Cytogenetic Analysis:	This is a hypotriploid human cell line with the modal chromosome number of 63 in 48% of cells examined. However, cells with 62 chromosome counts also occurred at a rather high frequency (24%). The rate of polyploidy was 1.6%., About 12 marker chromosomes are constantly found in most cells. They include: der(9)t(1;9)(q25;q34.3); del(1)(q25); der(13)t(11;13)(q13;q34); t(Xq1q); and eight others., At least two markers are found only in some cells. The normal Y chromosome was found in all cells. Only single copies of normal chromosomes 1, 10, 11 and 13 were present. Others were mostly in two or three copies per cell.
Age:	22 years
Gender:	male
Ethnicity:	Caucasian
Comments:	The NTERA-2 cl.D1 cell line is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line. [22741] The parental NTERA-2 lines was established in 1980 from a nude mouse xenograft of the Tera-2 cell line (see ATCC HTB-106). This clone differentiates along neuroectodermal lineages after exposure to retinoic acid (RA) or hexamethylene bisacetamide ((HMBA). [22321] [22999] The RA induced differentiation is characterized by glycolipid changes, appearance of neurons, and induction of homeobox (HOX) gene clusters. The cells exhibit high expression of N-myc oncogene activity. To induce differentiation, the cells should be trypsinized and seeded at a density 1 X 10 exp6 cells per 75 sq. cm. in medium containing 0.01 mM trans-retinoic acid. Stock solutions of trans-retinoic acid (10 mM, dissolved in DMSO) should be stored frozen (preferably under a nitrogen atmosphere).
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:	Medium Renewal: Every 2 to 3 days Subcultures are prepared by scraping. Cells from confluent cultures (approximately 20 million cells per 75 sq. cm.) are dislodged from the flask surface, aspirated and dispensed into new flasks. Cultures should be maintained at high density. Seed new flasks at a density of at least 5 X 10 exp6 viable cells per 75 sq. cm. flask.
Preservation:	culture medium 95%; DMSO, 5%
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020
References:	22321: Andrews PW. Human teratocarcinomas. Biochim. Biophys. Acta 948: 17-36, 1988. PubMed: 3293662 22336: Mavilio F, et al. Activation of four homeobox gene clusters in human embryonal carcinoma cells induced to differentiate by retinoic acid. Differentiation 37: 73-79, 1988. PubMed: 2898410 22407: Fenderson BA, et al. Glycolipid core structure switching from globo- to lacto- and ganglio- series during retinoic acid-induced differentiation of TERA-2-derived human embryonal carcinoma cells. Dev. Biol. 122: 21-34, 1987. PubMed: 3297853 22741: Andrews PW, et al. Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro. Lab. Invest. 50: 147-162, 1984. PubMed: 6694356 22746: Andrews PW, et al. Different patterns of glycolipid antigens are expressed following differentiation of TERA-2 human embryonal carcinoma cells induced by retinoic acid, hexamethylene bisacetamide (HMBA) or bromodeoxyuridine (BUdR). Differentiation 43: 131-138, 1990. PubMed: 2373286 22947: Gonczol E, et al. Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells. Science 224: 159-161, 1984. PubMed: 6322309 22999: Andrews PW. Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell line in vitro. Dev. Biol. 103: 285-293, 1984. PubMed: 6144603 23235: Hirka G, et al. Differentiation of human embryonal carcinoma cells induces human immunodeficiency virus permissiveness which is stimulated by human cytomegalovirus coinfection. J. Virol. 65: 2732-2735, 1991. PubMed: 1850047 29000: Dewji NN, Singer SJ. Cell surface expression of the Alzheimer disease-related presenilin proteins. Proc. Natl. Acad. Sci. USA 94: 9926-9931, 1997. PubMed: 9275228 49353: Baldassarre G, et al. Transfection with a CRIPTO anti-sense plasmid suppresses endogenous CRIPTO expression and inhibits transformation in a human embryonal carcinoma cell line. Int. J. Cancer 66: 538-543, 1996. PubMed: 8635871

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CRL-1973 NTERA-2 人恶性多发性畸胎瘤细胞