您好,欢迎来到仪表展览网!
请登录
免费注册
分享
微信
新浪微博
人人网
QQ空间
开心网
豆瓣
会员服务
进取版
标准版
尊贵版
|
设为首页
|
收藏
|
导航
|
帮助
|
移动端
|
官方微信扫一扫
微信扫一扫
收获行业前沿信息
产品
资讯
请输入产品名称
噪声分析仪
纺织检测仪器
Toc分析仪
PT-303红外测温仪
转矩测试仪
继电保护试验仪
定氮仪
首页
产品
知识
专题
词条
品牌
资料
展会
成功案例
网上展会
词多 效果好 就选易搜宝!
上海复祥生物科技有限公司
新增产品
|
公司简介
注册时间:
2010-01-11
联系人:
电话:
Email:
首页
公司简介
产品目录
公司新闻
技术文章
资料下载
成功案例
人才招聘
荣誉证书
联系我们
产品目录
黑胶虫
ATCC菌株_菌种库_微生物菌种
ATCC 菌种_质控菌株_标准菌株
微生物菌种_CMCC菌株
ATCC细胞、细胞株、肿瘤细胞
ATCC细胞
细胞株_人类正常细胞系目录
肿瘤细胞_人类肿瘤细胞系目录
细胞系_小鼠正常细胞系目录
肿瘤细胞株_小鼠肿瘤细胞系目录
细胞_大鼠正常细胞系目录
细胞购买_大鼠肿瘤细胞系目录
其它动物细胞系目录
美国PrimCells公司细胞
美国National Diagnostics公司试剂
美国National Diagnostics公司 Electrophoresis 电泳试剂
美国National Diagnostics公司 Histology 组织学试剂
美国National Diagnostics公司 Oxygen Assay 活性氧检测试剂
美国National Diagnostics 公司 Liquid Scintillation 液体闪烁试剂
美国National Diagnostics公司 Electro-Optical 电子光学试剂
血清
Hyclone 血清
gibco 血清
细胞因子
重组人细胞因子
重组动物细胞因子
Drierite干燥剂(无水硫酸钙)
脱蜡剂 透明剂
ELISA试剂盒
其它动物ELISA试剂盒
小鼠ELISA试剂盒
大鼠ELISA试剂盒
人ELISA试剂盒
**脂肪醇
动物疫病试剂盒
畜禽**检测试剂盒
宠物**检测试剂盒
快速检测卡
兽药残留检测卡
****检测卡
动物疫病检测卡
当前位置:
首页
>>>
产品目录
>>>
ATCC细胞、细胞株、肿瘤细胞
>>> 
肿瘤细胞株_小鼠肿瘤细胞系目录
仪���展览网
>>>
展馆展区
>>>
CRL-2586 EOMA 小鼠血管内皮瘤细胞
>
CRL-2586 EOMA 小鼠血管内皮瘤细胞
产品资料
CRL-2586 EOMA 小鼠血管内皮瘤细胞
如果您对该产品感兴趣的话,可以
产品名称:
CRL-2586 EOMA 小鼠血管内皮瘤细胞
产品型号:
CRL-2586
产品展商:
美国标准生物品收藏中心(ATCC)
产品文档:
无相关文档
简单介绍
CRL-2586 EOMA 小鼠血管内皮瘤细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件,
产品描述
CRL-2586 EOMA 小鼠血管内皮瘤细胞
ATCC
®
Number:
CRL-2586™
Price:
$329.00
Designations:
EOMA
Depositors:
R Auerbach
Biosafety Level:
1
Shipped:
frozen
Medium & Serum:
See Propagation
Growth Properties:
adherent
Organism:
Mus musculus
(mouse)
Morphology:
endothelial
Source:
Disease:
hemangioendothelioma
Strain:
129
Cell Type:
endothelial
Cellular Products:
angiotensin converting enzyme (ACE) [51514]
thrombospondin [51514]
cathepsin L [47148]
endostatin [47148]
interleukin-6 (interleukin 6, IL-6) [53484]
Permits/Forms:
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation:
Isolation date:
1980
Receptors:
acetylated low density liproprotein [51514]
Tumorigenic:
Yes
Antigen Expression:
CD31 +
vascular addressin +
CD45 (Ly5-T200) +
Age:
*****
Comments:
The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an ***** mouse. [51514]
The cells synthesize angiotensin-converting enzyme, express surface receptors for acetylated low density lipoprotein, produce thrombospondin and show intracellular staining with an antibody to von Willebrand factor. [51514]
Cathepsin L is secreted by EOMA cells and is responsible for the generation of endostatin L. [47148]
Although constitutive cytokine gene expression exists in EOMA cells, the level of IL-6 mRNA is prominently elevated by incubation with Liposome encapsulated hemoglobin (LEH). [53484]
The cells constitutively express the vascular addressin identified by antibody MECA-99.
EOMA cells exhibit characteristic endothelial cell properties, such as rearrangement into tubelike structures on Matrigel and retention of cobblestone morphology at confluence. They behave in vitro in a manner similar to microvascular endothelial cells. [51514]
Propagation:
ATCC complete growth medium:
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature:
37.0°C
Subculturing:
Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio:
A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal:
Every 2 to 3 days
Preservation:
Freeze medium:
Complete growth medium supplemented with 5% DMSO
Storage temperature:
liquid nitrogen vapor phase
Related Products:
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
derivative:ATCC CRL-2587
References:
47148: Felbor U, et al. Secreted cathepsin L generates endostatin from collagen XVIII. EMBO J. 19: 1187-1194, 2000. PubMed: 10716919
51514: Obeso J, et al. A hemangioendothelioma-derived cell line: its use as a model for the study of endothelial cell biology. Lab. Invest. 63: 259-269, 1990. PubMed: 2166185
53484: Zhu XL, et al. Kinetics of cytokine gene expression in macrophage and endothelial cell lines following liposome encapsulated haemoglobin (LEH) treatment in vitro. Cytokine 8: 541-547, 1996. PubMed: 8891435
53486: Wen W, et al. The generation of endostatin is mediated by elastase.. Cancer Res. 59: 6052-6056, 1999. PubMed: 10626789
ATCC® Number: CRL-2586™ Price: $329.00
Designations: EOMA
Depositors: R Auerbach
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: endothelial
Source: Disease: hemangioendothelioma
Strain: 129
Cell Type: endothelial
Cellular Products: angiotensin converting enzyme (ACE) [51514]
thrombospondin [51514]
cathepsin L [47148]
endostatin [47148]
interleukin-6 (interleukin 6, IL-6) [53484]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1980
Receptors: acetylated low density liproprotein [51514]
Tumorigenic: Yes
Antigen Expression: CD31 +
vascular addressin +
CD45 (Ly5-T200) +
Age: *****
Comments: The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an ***** mouse. [51514]
The cells synthesize angiotensin-converting enzyme, express surface receptors for acetylated low density lipoprotein, produce thrombospondin and show intracellular staining with an antibody to von Willebrand factor. [51514]
Cathepsin L is secreted by EOMA cells and is responsible for the generation of endostatin L. [47148]
Although constitutive cytokine gene expression exists in EOMA cells, the level of IL-6 mRNA is prominently elevated by incubation with Liposome encapsulated hemoglobin (LEH). [53484]
The cells constitutively express the vascular addressin identified by antibody MECA-99.
EOMA cells exhibit characteristic endothelial cell properties, such as rearrangement into tubelike structures on Matrigel and retention of cobblestone morphology at confluence. They behave in vitro in a manner similar to microvascular endothelial cells. [51514]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!
温馨提示:为规避购买风险,建议您在购买前务必确认供应商资质与产品质量。
免责申明:以上内容为注册会员自行发布,若信息的真实性、合法性存在争议,平台将会监督协助处理,
欢迎举报
产品留言
标题
内容
联系人
联系电话
电子邮件
公司名称
联系地址
验证码
点击换一张
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!
相关产品
LLC 小鼠Lewis肺癌细胞 LLC
CRL-2836 K7M2 wt 小鼠骨肉瘤成骨细胞 CRL-2836
3LL 3LL
Hepa1-6 小鼠肝癌细胞;Hepa1-6
J774A1小鼠单核巨噬细胞 J774A.1
若网站内容侵犯到您的权益,请通过网站上的联系方式及时联系我们修改或删除
沪公网安备 31010902002429号