Hut-102 Hut-102 人T**瘤细胞系 TIB-162

如果您对该产品感兴趣的话，可以
产品名称：Hut-102 Hut-102 人T**瘤细胞系
产品型号：TIB-162
产品展商：美国标准生物品收藏中心（ATCC）
产品文档：无相关文档

简单介绍

Hut-102 Hut-102 人T**瘤细胞系，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！

产品描述

Hut-102 Hut-102 人T**瘤细胞系

ATCC^® Number:

TIB-162™

Price:

$329.00

Designations:	HuT 102
Depositors:	AF Gazdar
Biosafety Level:	2 [Cells Contain RETROVIRUS ]
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	suspension, multicell aggregates
Organism:	Homo sapiens (human)
Morphology:	lymphoblast
Source:	Disease: lymphoma; mycosis fungoides Cell Type: cutaneous T lymphocyte;
Cellular Products:	HTLV-I (human T cell leukemia virus)
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors:	interleukin 2 (IL-2)
Antigen Expression:	CD4; Homo sapiens
DNA Profile (STR):	Amelogenin: X,Y CSF1PO: 8,11 D13S317: 11,13 D16S539: 12 D5S818: 8,13 D7S820: 8,10 THO1: 7,8 TPOX: 6 vWA: 16,19
Age:	26 years
Gender:	male
Ethnicity:	Black
Comments:	The Hut 102 cell line was derived from the peripheral blood of a 26 year old Black male patient with mycosis fungoides. This line has the properties of a mature human T cell with helper/inducer activity. It has receptors for IL-2, and the growth rate is stimulated by IL-2. The line is E-rosette positive (E+), and negative for Epstein-Barr virus nuclear antigen (EBNA-), surface immunoglobulin (sIg-) and terminal deoxynucleotidyl transferase (TdT-). The cells release a type C retrovirus that has been associated with human T cell lymphomas and should be handled as a biohazard. The line was originally thought to produce IL-2; however, recent studies have failed to detect IL-2 mRNA production in Hut 102 cells.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:	Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.Maintain cell density between 2 X 10 exp5 and 8 X 10 exp5 viable cells/ml. Do not allow density to exceed 1 X 10 exp6 cells/ml. Background debris is often present. Medium Renewal: Every 2 to 3 days
Preservation:	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature
Doubling Time:	about 41 hrs
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020
References:	1140: Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346 22610: Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013 23184: Hsu SM, et al. Expression of p55 (Tac) interleukin-2 receptor (IL-2R), but not p75 IL- 2R, in cultured H-RS cells and H-RS cells in tissues. Am. J. Pathol. 136: 735-744, 1990. PubMed: 1691591 23271: Poiesz BJ, et al. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc. Natl. Acad. Sci. USA 77: 7415-7419, 1980. PubMed: 6261256 23311: Wano Y, et al. Stable expression of the tax gene of type I human T-cell leukemia virus in human T cells activates specific cellular genes involved in growth. Proc. Natl. Acad. Sci. USA 85: 9733-9737, 1988. PubMed: 3059351

产品留言

标题

联系人

联系电话

内容

验证码

点击换一张

注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

温馨提示：为规避购买风险，建议您在购买前务必确认供应商资质与产品质量。
免责申明：以上内容为注册会员自行发布，若信息的真实性、合法性存在争议，平台将会监督协助处理，欢迎举报

产品目录

产品资料

Hut-102 Hut-102 人T**瘤细胞系