CRL-2630 Farage 人B**瘤细胞系

如果您对该产品感兴趣的话，可以
产品名称：CRL-2630 Farage 人B**瘤细胞系
产品型号：CRL-2630
产品展商：美国标准生物品收藏中心（ATCC）
产品文档：无相关文档

简单介绍

CRL-2630 Farage 人B**瘤细胞系，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和Z优培养条件！

产品描述

CRL-2630 Farage 人B**瘤细胞系

ATCC^® Number:

CRL-2630™

Price:

$338.00

Designations:	Farage
Depositors:	H Ben-Bassat
Biosafety Level:	2 [Cells contain Epstein-Barr virus (EBV) ]
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	suspension
Organism:	Homo sapiens (human)
Morphology:	lymphoblast
Source:	Disease: non-Hodgkin's B cell lymphoma Derived from metastatic site: lymph node Cell Type: B lymphocyte;
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression:	CD10 +/-; CD11a + (LFA-1); CD19 +; CD20 +; CD21 +; CD22 +; CD23 +; CD29 (VLA-4) +; CD38 +; CD39 +; CD40 +; CD44 +; CD54 + (ICAM-1); CD58 + (LFA-3); CD23 -; HLA DR + [55508] [53289]
DNA Profile (STR):	Amelogenin: X CSF1PO: 11,12 D13S317: 11,13 D16S539: 11,12 D5S818: 12 D7S820: 12 THO1: 8,9 TPOX: 9 vWA: 14,15
Cytogenetic Analysis:	trisomy of chromosome 11 [53289]
Age:
Gender:	female
Ethnicity:	White
Comments:	The Farage cell line was adapted to culture in 1990 from a lymph node biopsy of a patient with diffuse large cell non-Hodgkin's lymphoma (DLCL). [55508] The cells do not express surface or cytoplasmic immunoglobulin. [55508] Exposure to IL-4 augmented the concentrations of CD23, CD54, and CD58 but diminished the expression of CD21, CD22, and CD38. Incubation with IL-4 for 6 to 8 days led to increased expression of CD11a, CD39, CD40, and to disappearance of CD21 and CD38. [55510] [55508] Exposure of Farage cells to phorbol 12-myristate 13-acetate (PMA) down-regulated CD21 and CD23 expression. [55509] They do not express the terminal deoxynucleotydyl transferase gene (TdT), nor the recombination activating genes RAG-1 and RAG-2, known as markers of the pre-B cell stage. These results show that Farage represents a mature B-cell rather than a pre-B cell. [53288] The cells are positive for Epstein-Barr virus (EBV). [53289] mature B cell
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:	Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 - 5 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 3 X 10 exp5 and 3 X 10 exp6 viable cells/ml.
Preservation:	culture medium 95%; DMSO, 5%
Doubling Time:	24 to 36 hrs
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020
References:	53288: Baruch M, et al. Molecular characterization of an unusual non-Hodgkin's B-lymphoma cell line ("Farage") lacking the ability to produce immunoglobulin polypeptide chains. Leuk. Lymphoma 21: 485-495, 1996. PubMed: 9172815 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 55508: Shubinsky G, et al. The effect of IL-4 on the phenotype of a human B-cell lymphoma line (Farage) lacking immunoglobulin expression. Immunol. Lett. 36: 37-42, 1993. PubMed: 8344715 55509: Shubinsky G, et al. Pathways controlling the expression of surface CD21 (CR2) and CD23 (Fc(epsilon)IIR) proteins in human malignant B cells. Leuk. Lymphoma 25: 521-530, 1997. PubMed: 9250823 55510: Shubinsky G, Schlesinger M. Kinetics of the pleiotropic effect of interleukin 4 on the surface properties of human B-lymphoma cells. Leuk. Lymphoma 15: 333-340, 1994. PubMed: 7866283 55511: Shubinsky G. The mechanism of interleukin 4-induced down-regulation of CD38 on human B cells. Cell. Immunol. 10: 87-95, 1996. PubMed: 8871604

产品留言

标题

联系人

联系电话

内容

验证码

点击换一张

注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

温馨提示：为规避购买风险，建议您在购买前务必确认供应商资质与产品质量。
免责申明：以上内容为注册会员自行发布，若信息的真实性、合法性存在争议，平台将会监督协助处理，欢迎举报

产品目录

产品资料

CRL-2630 Farage 人B**瘤细胞系