CRL-2233 SNU-398 人肝癌细胞

如果您对该产品感兴趣的话，可以
产品名称：CRL-2233 SNU-398 人肝癌细胞
产品型号：SNU-398
产品展商：美国标准生物品收藏中心（ATCC）
产品文档：无相关文档

简单介绍

CRL-2233 SNU-398 人肝癌细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！

产品描述

CRL-2233 SNU-398 人肝癌细胞

ATCC^® Number:

CRL-2233™

Price:

$417.00

Designations:	SNU-398
Depositors:	J Park
Biosafety Level:	2 [CELLS CONTAIN HEPATITIS B VIRUS ]
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	mixed adherent and floating
Organism:	Homo sapiens (human)
Morphology:	epithelial
Source:	Organ: liver Disease: hepatocellular carcinoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions:	This line is available under the following restrictions: 1.) The cell line was deposited for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. The cells are provided as a service to the research community. They are provided without warranty or merchantability of fitness for a particular purpose or any other warranty, express or implied. 2.) Any proposed commercial use of these cells or products produced by them must first be negotiated with Jae-GaHB-Park, Director, Korean Cell Line Bank, 28 Yongon-dong, Chongno-gu, Seoul, 110-744 Korea. Telephone (02) 760-3380, Fax (02) 742-4727. 3.) In all papers reporting any use of these cells or derived products, a direct reference will be made to the original publication (Int. J. Cancer 62:276-282, 1995).
Antigen Expression:	Blood Type O; Rh +
DNA Profile (STR):	Amelogenin: X,Y CSF1PO: 13 D13S317: 11 D16S539: 10,14 D5S818: 12 D7S820: 10,11 THO1: 7,9 TPOX: 11 vWA: 17,18
Cytogenetic Analysis:	aneuploid; modal number = 62
Age:	42 years
Gender:	male
Ethnicity:	Asian
Comments:	SNU-398 was derived in 1990 by J.-G. Park and associates from an anaplastic hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a combination of doxorubicin and mitomycin-C. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum. Grossly, the original tumor was single nodular with perinodular extensions. Histologically, it was trabecular type. The cultured cells are multinucleated, and maintain the production of intracytoplasmic hyaline globules as seen in the original tumor. Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.
Propagation:	ATCC complete growth medium: RPMI 1640 medium, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation:	Culture medium, 95%; DMSO, 5%
Doubling Time:	39 hrs
References:	22872: Park JG, et al. Characterization of cell lines established from human hepatocellular carcinoma. Int. J. Cancer 62: 276-282, 1995. PubMed: 7543080

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products

产品留言

标题

联系人

联系电话

内容

验证码

点击换一张

注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

温馨提示：为规避购买风险，建议您在购买前务必确认供应商资质与产品质量。
免责申明：以上内容为注册会员自行发布，若信息的真实性、合法性存在争议，平台将会监督协助处理，欢迎举报

产品目录

产品资料

CRL-2233 SNU-398 人肝癌细胞