ones for further processing.
Results The SUPR-DSF produced high quality melting curves for all 16 samples, which included a Wild Type (WT) protein and 15 single-point mutation variants. The calculated melting temperatures agreed to within 0.2°C for the triplicate data. The fraction unfolded curves for all 15 mutants (Orange) compared to WT (Blue) are in Figure 1. Three of the mutants (9, 13, and 14) decreased the stability of the WT protein, which is easily seen by the shift to the left in the melting curve. The remaining 12 mutants all increased the stability of WT protein with Mutants 1, 8, and 12 showing the largest increase in stability of WT protein with Mutants 1, 8, and 12 showing the largest increase in stability
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