丙酮酸盐在糖酵解等过程中有重要作用: 它可以在细胞溶质葡萄糖进行糖酵解的过程中,由磷酸烯醇式丙酮酸产生。丙酮酸盐可以继续进入柠檬酸循环继续氧化分解,转化为碳水化合物或通过乙酰CoA 转化为脂肪酸。高水平的丙酮酸盐提示肝脏疾bing和遗传性代谢紊乱。丙酮酸盐也被用来作为减fei刺激剂。在无氧呼吸中丙酮酸可以转化为乳酸或者乙醇。
作为专业性的产品供应公司,艾美捷科技为您推荐,可同时用于比色法和荧光法的丙酮酸定量分析试剂盒:K609-100
产品名称及描述
货号
产品说明
Pyruvate Colorimetric/Fluorometric Assay Kit
K609-100
详情
* 细胞或组织样品需做去蛋白处理(会消耗丙酮酸),详情请见说明书 * 基于客户仪器设备,自行选择比色法或者荧光法即可
丙酮酸检测分析试剂盒原理: 丙酮酸盐检测试剂盒提供了一种简单、直接和可自动化操作的程序来测量不同生物学标本中丙酮酸的含量。适用标本类型包括:食物、细胞、培养基和发酵培养基。丙酮酸盐检测试剂盒基本原理如下:丙酮酸盐被丙酮酸酶氧化,在酶反应过程中产生颜色(λ= 570 nm)或者荧光(Ex/Em = 535/587 nm),可使用酶标仪或荧光计检测到。光的强度与丙酮酸含量是成正比的,因此通过检测光的强度即可**的得到丙酮酸的含量。试剂盒检测范围为1-10 uM丙酮酸。
《比色法和荧光法标准曲线展示》
丙酮酸检测分析试剂盒发表文章: 1.Alenzi, Mohammed et al. (2017) Antiurolithic effect of olive oil in a mouse model of ethylene glycol-induced urolithiasis, Investig Clin Urol. 2017 May;58(3):210-216. 2.Chem et al., Mitochondrial Citrate Transporter-dependent Metabolic Signature in the 22q11.2 Deletion Syndrome. J. Biol. Chem., Sep 2015; 290: 23240 - 23253. 3.Chen et al., Cadmium toxicity induces ER stress and apoptosis via impairing energy homoeostasis in cardiomyocytes. Biosci. Rep., Jun 2015; 35: e00214. 4.Chettimada et al., Hypoxia-induced glucose-6-phosphate dehydrogenase overexpression and -activation in pulmonary artery smooth muscle cells: implication in pulmonary hypertension.Am J Physiol Lung Cell Mol Physiol, Feb 2015; 308: L287 - L300. 5.Cho, S.C. et. al. DDS, 4,4'-diaminodiphenylsulfone, extends organismic lifespan. PNAS, Nov 2010; 107: 19326 - 19331. 6.Luitpold Fried et al., Identification of a Target Gene and Activating Stimulus for the YpdA/YpdB Histidine Kinase/Response Regulator System in Escherichia coli. J. Bacteriol., Feb 2013; 195: 807 - 815. 7.McNeely, K. et al. Redirecting Reductant Flux into Hydrogen Production via Metabolic Engineering of Fermentative Carbon Metabolism in a Cyanobacterium. Appl. Envir. Microbiol., 2010; 10.1128/AEM.00862-10 8.Min You et al., Ethanol administration exacerbates the abnormalities in hepatic lipid oxidation in genetically obese mice. Am J Physiol Gastrointest Liver Physiol, Jan 2013; 304: G38 - G47 9.Müller et al., Engineering of Ralstonia eutropha H16 for Autotrophic and Heterotrophic Production of Methyl Ketones. Appl. Envir. Microbiol., Jul 2013; 79: 4433 - 4439. 10.Negi et al., A Carbon-Nitrogen Lyase from Leucaena leucocephala Catalyzes the First Step of Mimosine Degradation. Plant Physiology, Feb 2014; 164: 922 - 934. 11.Odet, F. et. al. Lactate Dehydrogenase C and Energy Metabolism in Mouse Sperm. Biol Reprod, Sep 2011; 85: 556 - 564. 12.Palamiuc et al., A metabolic switch toward lipid use in glycolytic muscle is an early pathologic event in a mouse model of amyotrophic lateral sclerosis. EMBO Mol Med., May 2015; 7: 526 - 546. 13.Park et al., Changes in Pyruvate Metabolism Detected by Magnetic Resonance Imaging Are Linked to DNA Damage and Serve as a Sensor of Temozolomide Response in Glioblastoma Cells.Cancer Res., Dec 2014; 74: 7115 - 7124. 艾美捷科技与国内外优良的生物试剂供应商保持着密切的合作关系,目前已成为众多国际知名品牌的中国总代理或上等代理,主要包括:Abbkine、Merck Millipore、Origene、AAT Bioquest、Cayman Chemical、Abnova 、Biovision、ProSpec、Hycult Biotech 、Equitech-Bio、Trevigen、Alomone Labs、Epigentek、ImmunoReagents 、Jackson、SouthernBiotech、US Biological 、Caisson Labs等,可以在**时间为用户提供*前沿的专业资讯、完备的产品及物流服务。