Primary structure: One polypeptide chain (157 amino acids), identical to natural hTNF-α. Specific activity/EC50 : >1 x 10 8 U/mg, <0.01 ng/ml (hTNF-α, NIBSC, interim standard, 87/650), at least the same specific activity (EC50) compared to the indicated standard is guaranteed. EC50 definition/Unit definition: The amount of hTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.01 ng/ml). Species specificity: human, mouse
10 μg/ml solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane
Purity: >95% (SDS-PAGE) Endotoxin level: <0.1 EU/µg (LAL-test), <10 EU/ml (LAL-test) Note: 1 EU corresponds to 0.1 ng
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