SP6/T7 Transcription Kit Roche

In vitro transcription of DNA sequences cloned downstream of the SP6 or T7 promoter. Homogeneously labeled RNA can be synthesized with high efficiency (60 - 70% incorporation) using either radioactively (e.g., [³²P], [³H], [³⁵S]) labeled or nonradioactively (e.g., digoxigenin or biotin) labeled ribonucleotides.
Large amounts of RNA suitable for in vitro translation can also be produced.

1. pSPT18 DNA, 20 µl, 0.25 mg/ml
2. pSPT19 DNA, 20 µl, 0.25 mg/ml
3. Control DNA, 20 µl, 0.5 mg/ml
4. ATP, 40 µl, 10 mM
5. CTP, 40 µl, 10 mM
6. GTP, 40 µl, 10 mM
7. UTP, 40 µl, 10 mM
8. Transcription Buffer 10x, 100 µl
9. DNase I, RNase free, 200 U
10. RNase Inhibitor, 400 U
11. SP6 RNA Polymerase, 200 U
12. T7 RNA Polymerase, 200 U