Pierce Iminobiotin Agarose Pierce

Iminobiotin is the guanido analog of biotin. The dissociation constant of the avidin-iminobiotin complex is pH dependent. At pH 9.5-11.0, the avidin-iminobiotin complex will bind tightly. At pH 4, the avidin-iminobiotin complex will dissociate. Pierce has taken advantage of this interaction to create an immobilized iminobiotin support to bind avidin conjugates. Because no denaturing agents such as 8 M guanidine hydrochloride or 4 M urea are used in the purification, the avidin conjugate has a better chance of maintaining its activity during purification.

Highlights:

• Denaturing conditions not required for recovery of biotinylated molecule
• Support can be reused up to 10 times

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