BMH Pierce

Thermo Scientific Pierce BMH is a long-arm, maleimide crosslinker for covalent, irreversible conjugation between sulfhydryl groups (e.g., protein or peptide cysteines).

Highlights:

• Reactive groups: maleimide (homobifunctional)
• Reactive towards: sulfhydryl groups
• BMH permits irreversible crosslinking of sulfhydryl- containing compounds, using mild conditions
• Maleimides react specifically with sulfhydryl groups at pH 6.5-7.5
• Water insoluble; non-cleavable
• Mild, irreversible –SH to –SH crosslinking agent

References:

1. Chen, L.L., Rosa, J.J., Turner, S. and Pepinsky, R.B. (1991). Production of multimeric forms of CD4 through a sugar-based crosslinking strategy. J. Biol. Chem. 266(27), 18237-18243.
2. Hansen, M.S.T. and Barklis, E. (1995). J. Virol. 69(2), 1150-1159.
3. Ikushiro, S., et al. (1997). Biochemistry 36(23), 7154-7161.
4. Schmidt, C.J., et al. (1992). J. Biol. Chem. 267(20), 13807-13810.