BMDB Pierce

Thermo Scientific Pierce BMDB is a mid-length, periodate-cleavable, maleimide crosslinker for covalent but reversible conjugation between sulfhydryl groups (e.g., protein or peptide cysteines).

Highlights:

• Crosslinks sulfhydryl-containing compounds under mild conditions
• Specific reactivity with -SH groups at pH 6.5-7.5
• Crosslinks reversed by treatment with 15 mM sodium periodate
• Cleavable by Sodium meta-Periodate (Product # 20504)
• Reactive Groups: maleimide (homobifunctional)
• Reactive toward: sulfhydryl groups

References:

1. Stalteri, M.A. and Mather, S.J. (1995). Bioconj. Chem. 6, 179-186.
2. Yi, F., et al. (1991). J. Biol. Chem. 266(6), 3900-3906.
3. Hermanson, G.T. (1996). Bioconjugate Techniques, San Diego, Academic Press,
   pp. 114-116, 294. (periodate cleavage)
4. O’Sullivan, M.J., et al. (1979). Anal. Biochem. 100, 100-108.