Fluoraldehyde o-Phthalaldehyde Crystals Pierce

Thermo Scientific Pierce Fluoraldehyde (OPA) is o-phthalaldehyde, a highly sensitive fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC.

Highlights:

• A highly sensitive fluorescent pre- or post-column reagent for amino acid detection and quantitation
• Provides an accurate measure of both composition and absolute protein/peptide content
• Ideal when working with recombinant proteins, synthetic peptides and other “next generation” applications
• Reacts with all primary amine-containing analytes
• High sensitivity; low background
• Can be used for fluorescent protein or peptide assay
• Stable in aqueous solution

Product Details:

Figure 1. Excellent linear response. Fluoraldehyde Reagent Solution
shows excellent linear response, whether in the 2500 or 200 picomole range.

Figure 2. Outstanding shelf life. Comparison of fluorescence
response of selected amino acids after reaction with recently-
prepared and one-year-old Fluoraldehyde Reagent Solutions.

For even greater sensitivity, use a combination of OPA with Fmoc-Chloride with automated pre-column derivatization, detecting both primary and secondary amines. With this application, primary amino acids are first derivatized with OPA, while non-reacted secondary amino acids are then reacted with Fmoc-Chloride, resulting in extraordinary amino acid detection sensitivity and accuracy.^1,2

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