Fluoraldehyde Reagent Pierce

Thermo Scientific Pierce Fluoraldehyde (OPA) Reagent is a ready-to-use solution of o-phthalaldehyde, a fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC.

Highlights:

• A highly sensitive fluorescent pre- or post-column reagent for amino acid detection and quantitation
• Provides an accurate measure of both composition and absolute protein/peptide content
• Ideal when working with recombinant proteins, synthetic peptides and other “next generation” applications
• Reacts with all primary amine-containing analytes
• All primary amino acids react, resulting in highly fluorescent isoindole derivatives for HPLC analysis
• High sensitivity; low background
• Can be injected into your LC with no further processing
• Can be used for fluorescent protein or peptide assay
   

Product Details:

Figure 1. Excellent linear response. Fluoraldehyde Reagent Solution
shows excellent linear response, whether in the 2500 or 200 picomole range.

Figure 2. Outstanding shelf life. Comparison of fluorescence
response of selected amino acids after reaction with recently-
prepared and one-year-old Fluoraldehyde Reagent Solutions.

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