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赛多利斯Vivaflow50切向流过滤器

赛多利斯Vivaflow50切向流过滤器
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简单介绍
赛多利斯Vivaflow50切向流过滤器
产品描述

切向流浓缩器,特别适合于0.1L-5L生物样品和纳米粒子的超滤、浓缩和交换缓冲液。Vivaflow 50和Vivaflow 200是市场上**的

实验室回旋流/切向流超滤器。 Sartorius Stedim Biotech切向流超滤器能带动任何流速为200-400 ml/min的实验室蠕动泵。

 

Plug and play laboratory cross flow cassette
Modular - scalable to 6 units
Disposable
Volumes from 0.1 l -3 l
Optimal for aqueous sample concentration e.g. proteins and nanoparticles
High flow rate and recovery


Description

Vivaflow 50 is a disposable and ready-to-use crossflow cassette for filtration and concentration of samples from 100 ml to 3 l. The membrane surface per device is 50 cm2. One pack contains two devices and comes with all necessary accessories for running two devices with a laboratory pump and a size 16 pump head. For speeding up the concentration and increasing the volume throughput, six cassettes can be connected.

Optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breading facilities, this economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 50 cm2 module typically reduces 500 ml to 15 ml in less than 50 min. Nearly total recovery of the concentrate is achieved with a single rinse. Up to six of the unique interlocking modules can be connected for easy scale up resulting in a capacity of several liters. Vivaflow 50 is intended for single use. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.


设备需求

标配的Vivaflow 50包含两个超滤器、管路、流速限制器、一系列的联网和高压蠕动泵管。只需另配一台可使用16号泵管(6.4mm OD)的蠕动泵。

 

能同时行两个以上Vivaflow 50设备

为了加快浓缩和增加通量,可连接6个Vivaflow 50 超滤器,通过2个T型连接器在入口和出口处连接2个Vivaflow超滤器,再在设备之间进行一系列的联网即可。压力指示器只需在进口配置一个即可达到*佳控制效果。

 

高流速和高回收率
透明外壳,适合过程监控
即用型单个超滤器
可简单连接多个超滤器
经济实用、可抛弃型产品
独特的性能

 

有效膜面积为50 cm2的单个超滤器,将500ml样品浓缩至15ml以下,只需不到50分钟。*小循环体积小于10ml,样品可达到*大限度的浓缩。不能回收的残留体积小于1.5ml。用10ml缓冲液润洗一次,可达到近乎完全回收。

 

性能参数

 


3 bar进气压,20°C
浓缩至20倍 [分]时间
     
独立装置 Three devices 液体回收率 %
250 ml 起始体积. 分钟 1 L 起始体积. 分钟. Direct 10 ml rinse
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES 34 49 96 % > 99 %
10,000 MWCO PES 22 32 94 % > 99 %
30,000 MWCO PES 22 32 92 % 99 %
50,000 MWCO PES 20 29 92 % 98 %
γ Globulins 1.0 mg/ml
100,000 MWCO PES 43 62 92 % 98 %
100,000 MWCO RC 40 58 92 % 98 %
Yeast 1.0 mg/ml (S.Cerevisiae)
0.2 μm PES 33 47 92 % 98 %


产品特性

Market Research
应用 Concentration;Desalting | buffer exchange
Ultrafiltration device type Tangential | crossflow concentrator
样品类型 Nanoparticles;Peptide;Protein;Virus
MWCO 10.000 Dalton
Membrane Polyethersulfone (PES)
Type Tangential | crossflow concentrator
Volume Range 100 - 3.000 ml
Certification None

 

技术属性

Overall length 107 mm
Overall height 84 mm
Overall width 25 mm
Channel width 15 mm
Channel height 0,3 mm
Active Membrane Area 50 cm2
Hold-up volume 1,5 ml
Minimum Recirculation Volume < 10 ml
Non-recoverable Hold-up < 0,5 ml
Pump Flow 200 - 400 ml/min
Operating pressure max. 3 bar
Max. temperature 60 °C
Main Housing Polycarbonate (PC)
Flow Channel TPX (PMP)
Membrane support TPX (PMP)
Seals and O-rings Silicone
Pressure indicator Polypropylene, SS spring
Flow Restrictor Polypropylene (PP)
Fittings Nylon (NY)
Tubing PVC (medical grade)

 


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