Column:
Polysulfonix-SCX, 120 Å, 5 µm, 4.6 x 250 mm
Mobile phase:
A : B = 62 : 38 ( v/v ) A: ACN; B:Phosphoric acid sodium(2 →1000, Adjust pH 3.8 with Ammonia solution)
Flow rate:
0.5 mL/min
Temperature:
40 oC
Detection:
UV 215 nm
Injection volume:
10 µL
Sample:
1. Arecoline hydrobromide (0.0995 mg/mL)