BioAimScientific 1010008 人白细胞介素1-β快速ELISA试剂盒 人白细胞介素1-β试剂盒 Human IL-1 beta EasyTest™ ELISA Kit
Human IL-1 beta EasyTest™ ELISA KitHomeELISAEasyTest™ ELISA KitsHuman IL-1 beta EasyTest™ ELISA Kit
Human IL-1 beta EasyTest™ ELISA Kit
Human IL-1 beta ELISA Kit Standard Curve
Human IL-1 beta EasyTest™ ELISA Kit
Catalog No : 1010008
Sizes
$529.00
Product Features
Product Name Human IL-1 beta EasyTest™ ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Process One wash step, two incubations
Assay Length 1.5 hours
Sample Volume Serum , Plasma, 50µL
Detection Method Colorimetric
Sensitivity < 1pg/mL
Assay Range 8.2-2000pg/mL
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names IL1B; IL-1; IL1-BETA; IL1F2
Gene ID 3553
Swissprot ID P01584
Recovery
| Sample Type | Average % Recovery | Range % |
| Serum | 96 | 76-114 |
| Plasma | 84 | 78-92 |
Linearity
| Sample Type | Dilution | % of Expected |
| Serum | 1:2 | 94 |
| 1:4 | 129 | |
| 1:8 | 135 | |
| Plasma | 1:2 | 104 |
| 1:4 | 103 | |
| 1:8 | 122 |
Standard Curve: Human IL-1 beta EasyTest™ ELISA Kit
Kit Components
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human IL-1 beta mix
• TMB One-Step Substrate
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.
Background
IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. IL-1β is a member of the interleukin 1 family of cytokines. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 . This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 by this cytokine in the central nervous system is found to contribute to inflammatory pain hypersensitivity. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
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Human IL-1 beta ELISA Kit
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Regulatory Status
Human IL-1 beta EasyTest™ ELISA Kit is for Research Use Only.
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