bioaimscientific 1010012 人神经调节蛋白1-beta1快速ELISA试剂盒 Human NRG1-beta 1 EasyTest™ ELISA Kit
Human NRG1-beta 1 EasyTest™ ELISA KitHomeELISAEasyTest™ ELISA KitsHuman NRG1-beta 1 EasyTest™ ELISA Kit
Human NRG1-beta 1 EasyTest™ ELISA Kit
Human NRG1-beta 1 ELISA Kit Standard Curve
Human NRG1-beta 1 EasyTest™ ELISA Kit
Catalog No : 1010012
Sizes
$529.00
Product Features
Product Name Human NRG1-beta 1 EasyTest™ ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Process One wash step, two incubations
Assay Length 1.5 hours
Sample Volume Serum , Plasma, 50µL
Detection Method Colorimetric
Sensitivity 200pg/mL
Assay Range 937.5-60000pg/mL
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names NRG1; GGF; HGL; HRGA; NDF; SMDF
Swissprot ID Q02297
Recovery
| Sample Type | Average % Recovery | Range % |
| Serum | 123 | 118-131 |
| Plasma | 126 | 124-138 |
Linearity
| Sample Type | Dilution | % of Expected |
| Serum | 1:2 | 95 |
| 1:4 | 90 | |
| 1:8 | 96 | |
| Plasma | 1:2 | 92 |
| 1:4 | 83 | |
| 1:8 | 81 |
Standard Curve: Human NRG1-beta 1 EasyTest™ ELISA Kit
Kit Components
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human NRG1-beta 1 mix
• TMB One-Step Substrate
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.
Background
The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. All NRG1 isoforms contain an EGF-like domain that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. NRG1 isoforms can be classified into three major subtypes. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Regulatory Status
Human NRG1-beta 1 EasyTest™ ELISA Kit is for Research Use Only.
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