产品名称：Monoclonal Antibody to Human Factor B (Bb)

产品介绍
品名	Monoclonal Antibody to Human Factor B (Bb)
货号	A227
规格	100 µL
同种型	IgG1K
蛋白质浓度	> 1.0 mg/mL
纯度	≥ 95% by SDS PAGE
缓冲液	Borate Buffered Saline (pH 8.4 ± 0.2)
物种交叉反应	None tested.
储存	≤ 30 Days 2-8˚C  > 30 Days ≤ –20˚C
使用说明	For Research Use Only. Not for use in Diagnostic Procedures
背景	Factor B is one of the complement proteins which is unique to the Alternative Pathway. It is a single-chain glycoprotein with a molecular weight of approximately 98 kD. The concentration of Factor B in human serum/plasma is ~200 μg/ml.  The Alternative Complement Pathway provides innate protection against microbial agents in the absence of specific antibodies. The activation of this complement pathway can be triggered by a variety of substances, including microbial polysaccharaides or lipids, gram-negative bacterial lipopolysaccharides, and surface determinants present on some viruses, parasites, virally infected mammalian cells, and cancer cells. In autoimmune diseases, the Alternative Complement Pathway may contribute directly to tissue damage.
特异性	The specificity of the monoclonal antibody for the Factor B (Bb) protein was established by ELISA. The antibody was shown to bind highly purified Factor B and its Bb fragment, but not to the Ba fragment. The antibody binds a non-neoantigen in the Bb domain of native Factor B, since highly purified, functionally active Factor B and normal human plasma blocked binding of the antibody to both Factor B and Bb fragments coated in microtiter wells. Zymosan activated human serum was not more effective than the corresponding non-activated plasma in blocking the binding of this antibody.
应用
参考资料	1、Wright, A., Morrison, S. Effect of C2 Associated Carbohydrate structure of Ig Effector function: studies with chimeric mouse human IgG1 Antibodies in glycosylation mutants of chinese hamster ovary cells, J. Immunol 160:3393-3402, 1998. 2、Hourcade, D., et al. A conserved element in the serine protease domain of complement Factor B, J Bio Chem 273:25996-26000, 1998.