产品名称：Monoclonal Antibody to Human Factor I (#1)

产品介绍
品名	Monoclonal Antibody to Human Factor I (#1)
货号	A247
规格	100 µL
同种型	IgG1k
蛋白质浓度	> 1.0 mg/mL
纯度	≥ 95% by SDS PAGE
缓冲液	Borate Buffered Saline (pH 8.4 ± 0.2)
物种交叉反应	Human
储存	≤ 30 Days 2-8˚C  > 30 Days ≤ –20˚C
使用说明	For Research Use Only. Not for use in Diagnostic Procedures
产品描述	A murine monoclonal antibody to an epitope Factor I. This monoclonal antibody was raised against purified human Factor I. This antibody blocks function of Factor I. Quidel's Factor I monoclonal antibody (#1) has been used in a variety of experimental techniques including EIA, Western Blot, and RIA
背景	Factor I is one of the complement proteins which is unique to the Alternative Pathway. It is a 88 kD glycoprotein composed of heavy (50 kD) and light (38 kD) chains linked by disulphide bonds. Factor I is a highly specific serine protease which cleaves the alpha chain of C3b and C4b leading to the formation of iC3b and C3f, followed by C3c and C3d,g. It does not function effectively without a cofactor, such as Factor H, CR1, MCP or CR2. The concentration of Factor I in human serum/plasma is ~34 µg/ml.  The Alternative Complement Pathway provides innate protection against microbial agents in the absence of specific antibodies. The activation of this complement pathway can be triggered by a variety of substances, including microbial polysaccharaides or lipids, gram-negative bacterial lipopolysaccharides, and surface determinants present on some viruses, parasites, virally infected mammalian cells, and cancer cells. In autoimmune diseases, the Alternative Complement Pathway may contribute directly to tissue damage.
特异性	The specificity of the monoclonal antibody was established by ELISA and RIA. The antibody was shown to bind highly purified Factor I. By RIA, the antibody immunoprecipitates radiolabeled Factor I sing protein A-bearing bacteria to capture the immune complex. The antibody does not inhibit the cleavage of C3b to iC3b in the presence of Factor H and Factor I.
应用