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第12年
- 入驻时间： 2012-04-17
- 联系人：客户经理
- 电话：400-968-7988
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联系时，请说明易展网看到的
- Email：fuwu@bioleaf.com
产品详情
简单介绍：
CD4 Antibody (PE)
详情介绍：
Mouse monoclonal antibody to CD4 (PE).
| Target | CD4 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Tested Applications | IF/ICC, FCM |
| Purification | The antibody was purified by affinity chromatography. |
| Form | Liquid |
| Isotype | IgG2a |
| Conjugation | PE |
| Specificity | Recognises human CD4. |
| Storage | Shipped and store at 4°C for one year. Do not freeze. |
| Swiss Prot | P01730 |
| GeneID | 920 |
| Gene Symbol | CD4 |
| Buffer | PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide. |
| Directions for use | 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of a 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of the flow tube mixing with the whole blood; incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes away from light after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |
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