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产品介绍 |
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品名 |
Rabbit antiserum against calmodulin. |
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货号 |
465 |
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名称 |
200ul |
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形式 |
lyophilized whole serum (no preservatives). |
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复溶 |
with 200 µl bidistilled water |
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描述 |
This antibody was raised against performic acid-oxidized vertebrate calmodulin. It reacts preferentially with vertebrate calmodulin (chicken, bovine, rat, human) but shows minor cross-reactivity (<5%) with plant calmodulin (spinach). |
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稀释 |
Immunohistochemistry: 1: 1'000 for use with the avidin-biotin system. Radioimmunoassay: use at 1:50 to 1:100 dilution to detect a minimum of 1 ng calmodulin (10 ng/ml or 600 pM calmodulin by concentration). Western blot: 1:100 to 1:1'000 to detect down to 30 ng calmodulin on the gel. |
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储存 |
For long storage keep small aliquots of the reconstituted antiserum at minus 80°C (or at least at -20°C). Avoid repeated freeze-thaw cycles. |
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参考文献 |
L. Van Eldik and M. Watterson, J. Biol. Chem. 256: 4205-4210 (1981) |