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产品介绍 |
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品名 |
Rabbit anti-Tescalcin |
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货号 |
T876 |
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规格 |
200ul |
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克隆性 |
polyclonal |
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共轭 |
rabbit |
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反应性 |
nonconjugated |
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宿主动物 |
Rabbit |
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抗体形式 |
Antiserum lyophil. |
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产品形式 |
Lyophilized whole serum (no preservatives) |
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复溶 |
with 200 µl of bi-distilled water. |
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产品描述 |
The antiserum against Tescalcin is produced in rabbits by immunization with recombinant mouse Tescalcin containing a 6-his tag at the N-terminal. The antibody was evaluated for specificity and potency: a) by Biotin-Avidin labeling of cryostate-, vibratome- and paraffin-sections of 4% paraformaldehyde fixed brains and b) by immunoblots (Girard et al, 2015). |
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产品用途 |
Tescalcin is a novel calcineurin B-like protein that binds a single Ca2+ ion. It occurs mainly in the heart; stomach and brain (see also the Allen brain atlas (http://www.brainmap.org/). |
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稀释 |
Immunohistochemistry: 1:2'000 - 1:5'000, on paraformaldehyde (4%) or formalin-fixed tissue, cryostate or paraffin-sections. Immunoblots: 1:1'000 - 1:2'000. For immunohistochemistry and immunoblots the titre was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments. |
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储存 |
After reconstitution freeze in small aliquots (e.g. 1 µl) and keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing. |
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参考文献 |
Girard F, Venail J, Schwaller B, Celio MR. (2015) The EF-hand Ca(2+)-binding protein super-family: a genome-wide analysis of gene expression patterns in the a dult mouse brain.Neuroscience;294:116-55. |