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产品介绍 |
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| 品名 |
Rabbit anti-ALG2 8522 |
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| 货号 |
Mb-2 |
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| 规格 |
200 µl |
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| 宿主动物 | Rabbit | ||
| 抗体形式 |
Antiserum lyophil |
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| 产品形式 |
Lyophilized antiserum (no preservatives) |
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| 复溶 |
with 200 µl bidistilled water |
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| 描述 |
In many apoptotic pathways Ca2+ seems to play an important role. The Ca2+ binding
protein ALG-2 is an apoptotic protein discovered in a death trap assay and described
as a proapoptotic protein by the group of D'Adamio (1). The protein of 191 amino
acids contains two canonical Ca2+ binding EF-hand domains, binding Ca2+ with high
affinity and one non canonical EF-hand, binding Ca2+ with low affinity. Cells lacking
ALG-2 still contain active caspases but are protected against apoptosis induced by
Fas, glucocorticoids and staurosporin. The protein is expressed ubiquitously and was
conserved during evolution from C.elegans to man (2). Expression of ALG-2 is
increased in various cancers (3). This antiserum was made against the full-length
recombinant ALG2-protein. |
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| 稀释 |
Immunohistochemistry: 1:2'000 - 1:5'000 with the avidin-biotin method. On paraformaldehyde (4%) or formalin-fixed tissue. Cryostate sections. Immunoblots: 1:1’000 - 1:2'000. We recommend that the optimal dilutions be determined by titration experiments
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| 储存 |
After reconstitution freeze in small aliquots (e.g. 1 µl). For long storage, keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Naazide). Avoid repeated freezing and thawing |
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| 参考文献 |
1. Vito, P., Lacana, E. & D'Adamio, L. Science 271, 521-525 (1996). 2. Tarabykina, S., Læssø Møller, A., Durussel, I., Cox, J. and Berchtold, M.W., J. Biol. Chem. 275, 10514-10518 (2000). 3. La Cour JM, Moellerup J., Winding P., Tarabykina S., Sehested M. and Berchtold MW, Am. J. Pathol., 163, 81- 89 (2003).
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